|
KMID : 0043320090320020201
|
|
Archives of Pharmacal Research
2009 Volume.32 No. 2 p.201 ~ p.206
|
|
|
Simultaneous Determination of Quercetin and its Glycosides from the Leaves of Nelumbo nucifera by Reversed-Phase High-Performance Liquid Chromatography
|
|
Choi Jae-Sue
|
|
|
Abstract
|
|
|
|
The purpose of this study was to develop reversed-phase HPLC method for the simultaneous determination of the flavonoids from the leaves of Nelumbo nucifera, which have been known to exhibit antioxidant, anti-HIV, antihyperlipidemic and antiobesity effects. HPLC separation was achieved on C18 column using gradient elution with mobile phase consisting of acetonitrile-water containing 0.1% formicacid. The separated peaks were identified by electrospray ionization mass spectrometry. The HPLC method was validated and applied for the simultaneous determination of the bioactive flavonoids from the leaves of Nelumbo nucifera. In the methanol extract, six flavonoids, quercetin, rutin, quercetin 3-O-β-D-galactopyranoside (Qc-3-Gal), quercetin 3-O-β-D-glucopyranoside (Qc-3-Glc), quercetin 3-O-β-Dglucuronide (Qc-3-Gln) and quercetin 3-O-α-arabinopyranosyl-(1→2)-β alactopyranoside (Qc-3-AraGal), were identified. Among them, Qc-3-Glc and Qc-3-Gln were found to be major component in the methanol extract of Nelumbo nucifera leaves.
|
|
|
KEYWORD
|
|
|
Quercetin, Quercetin glycoside, Flavonoid, Nelumbo nucifera, Reversed-phase HPLC
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|